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They face specific challenges concerning cold-chain storage requirements and potential instability [231]

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That just reintroduces contaminants

Strep-MBP-GRX samples were reduced with 10 mM DTT for 2 hours and the buffer was exchanged against 100 mM Tris/HCl p H 8.0, 1 mM EDTA using the PD MiniTrap G-25 desalting columns (GE Healthcare)